Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples-Reference-Cited by-同舟云学术

Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples

Published:2008-07 Issue:7 Volume:46 Page:2195-2199
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chan Kwok Hung¹,Yam Wing Cheong¹,Pang Chiu Mei¹,Chan Kit Man¹,Lam Siu Yan¹,Lo Kam Fai¹,Poon Leo L. M.¹,Peiris J. S. Malik¹²

Affiliation:

1. Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong, SAR, People's Republic of China

2. HKU-Pasteur Research Centre, Sassoon Rd., Pokfualm, Hong Kong, SAR, People's Republic of China

Abstract

ABSTRACT The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00315-08

Reference18 articles.

1. Detection and Identification of Human Parainfluenza Viruses 1, 2, 3, and 4 in Clinical Samples of Pediatric Patients by Multiplex Reverse Transcription-PCR

2. Multiplex Real-Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses

3. Improved Silica-Guanidiniumthiocyanate DNA Isolation Procedure Based on Selective Binding of Bovine Alpha-Casein to Silica Particles

4. Cadieux, N., P. Lebel, and R. Brousseau. 1993. Use of a triplex polymerase chain reaction for the detection and differentiation of Mycoplasma pneumoniae and Mycoplasma genitalium in the presence of human DNA. J. Gen. Microbiol.139:2431-2437.

5. Evaluation of the Directigen FluA+B Test for Rapid Diagnosis of Influenza Virus Type A and B Infections

Cited by 43 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Viral infection in chronic otitis media with effusion in children;Frontiers in Pediatrics;2023-05-10

2. Importance of sample input volume for accurate SARS-CoV-2 qPCR testing;Analytica Chimica Acta;2022-03

3. Use of MALDI-TOF mass spectrometry for virus identification: a review;The Analyst;2022

4. Biorobots;Machine Learning in Biological Sciences;2022

5. Evaluation of the molecular Xpert Xpress Flu/RSV assay vs. Alere i Influenza A & B assay for rapid detection of influenza viruses;Diagnostic Microbiology and Infectious Disease;2018-03