Abstract
The complete nucleotide sequence of a molecular clone of Moloney murine leukemia virus (pMLV-1) has previously been reported (Shinnick et al., Nature [London] 293:543-548, 1981). However, pMLV-1 does not generate infectious virus after transfection into cells (Berns et al., J. Virol. 36:254-263, 1980). The lesion in pMLV-1 has been localized by determining the biological activity of recombinants containing DNA from an infectious clone of Moloney murine leukemia virus (pMLV-48) and pMLV-1. Replacement of a 1.0-kilobase pair region which spans the gag-pol junction of pMLV-1 with the corresponding DNA fragment from the infectious clone restores its infectivity. Nucleotide sequence analysis of this fragment obtained from the infectious clone (pMLV-48) and pMLV-1 reveals two single base pair changes, one in the p30gag gene and the other in the 5' end of the pol gene. The mutation in the pol gene does not affect the production of infectious virus but renders them XC negative, whereas the mutation in the gag gene appears to be lethal. The complete nucleotide sequence of an infectious clone of Moloney murine leukemia virus can now be deduced.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference31 articles.
1. Isolation of recombinant DNA clones carrying complete integrated proviruses of Moloney murine leukemia virus;Bacheler L.;J. Virol.,1981
2. Molecular cloning of unintegrated and a portion of integrated Moloney murine leukemia viral DNA in bacteriophage lambda;Berns A. J. M.;J. Virol.,1980
3. Enhancing the efficiency of DNA-mediated gene transfer in mammalian cells;Corsaro C. M.;Somatic Cell Genet.,1981
4. Dickson C. R. Eisenman H. Fan E. Hunter and N. Teich. 1982. Protein biosynthesis and assembly p. 513-648. In R. A. Weiss N. Teich H. E. Varmus and J. M. Coffin (ed.) Molecular biology of tumor viruses part III. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
5. Host restriction of Friend leukemia virus: gag proteins of host range variants;Duttagupta S.;Proc. Natl. Acad. Sci. U.S.A.,1981
Cited by
66 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献