Transcriptional mapping of the DNA polymerase gene of vaccinia virus

Author:

Traktman P,Sridhar P,Condit R C,Roberts B E

Abstract

Vaccinia virus DNA polymerase, a single-subunit enzyme of 110,000 molecular weight, is induced early after infection. Genetic analysis suggests that the gene encoding the enzyme maps within a 15-kilobase HindIII fragment located 45 kilobases from the left-hand end of the genome. We identified the in vitro translation product with these properties and mapped the transcript by hybrid selection, RNA filter hybridization, and S1 nuclease mapping. Two mRNAs from this region, 3.4 and 3.9 kilobases in size, could be translated in vitro to yield a 110K polypeptide. The two RNAs shared a common 5' terminus and had staggered 3' ends. Sequences mapping entirely within this gene were shown to be biologically active in rescuing mutants with temperature-sensitive or drug-resistant polymerase activity to the wild-type phenotype.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference27 articles.

1. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes;Alwine J. D.;Proc. Natl. Acad. Sci. U.S.A.,1979

2. The isolation and characterization of Drosophila yolk protein genes;Barnett T.;Cell,1980

3. Cell-free translation of early and late mRNAs selected by hybridization to cloned DNA fragments from the left 14 million to 72 million daltons of the vaccinia virus genome;Belle-Isle H.;Virology,1981

4. Ultraviolet mapping of the adenovirus 2 early promoters;Berk A. J.;Cell,1977

5. Separation of a new deoxyribonucleic acid polymerase from vacciniainfected HeLa cells;Berns K. J.;J. Virol.,1969

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