Modified quantitative association of official analytical chemists sporicidal test for liquid chemical germicides

Author:

Miner N,Armstrong M,Carr C D,Maida B,Schlotfeld L

Abstract

The Association of Official Analytical Chemists (AOAC) test for sporicidal activity of disinfectants (966.04) is used in the United States as the legal criteria for classifying a liquid as a chemical sterilant and also as the indicator of the highest level of disinfectant. This qualitative test contains procedures that may cause inaccurate results. A modified, quantitative version of the AOAC sporicidal test has been developed that uses a specified minimum number of spores (2.0 x 10(sup5)) of Bacillus subtilis or Clostridium sporogenes dried onto porcelain penicylinders. This modified test was validated with three commercial sterilant chemicals tested on three separate groups of spore-labeled cylinders.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference16 articles.

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2. Beloian A. 1995. Disinfectants p. 12-13. In P. Cunniff (ed.) Official methods of analysis of AOAC International 16th ed. Association of Official Analytical Chemists Arlington Va.

3. Beloian A. 1983. Methods of testing for sterility: efficacy of sterilizers sporicides and sterilizing processes p. 885-917. In S. S. Block (ed.) Disinfection sterilization and preservation 3rd ed. Lea & Febiger Philadelphia Pa.

4. Borick P. M. and R. E. Pepper. 1970. Disinfection p. 85-102. Marcel Dekker Inc. New York N.Y.

5. Evaluation of microbial loads of Bacillus subtilis spores on penicylinders;Danielson J. W.;J. AOAC Int.,1993

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