High-Resolution Physical Map of the Sinorhizobium meliloti 1021 pSyma Megaplasmid-Reference-Cited by-同舟云学术

High-Resolution Physical Map of the Sinorhizobium meliloti 1021 pSyma Megaplasmid

Published:2000-02-15 Issue:4 Volume:182 Page:1185-1189
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Barloy-Hubler Frederique¹,Capela Delphine¹²,Barnett Melanie J.³,Kalman Sue⁴,Federspiel Nancy A.⁴,Long Sharon R.³⁵,Galibert Francis¹

Affiliation:

1. Laboratoire de Recombinaisons Génétiques UPR41-CNRS, Faculté de Médecine, F-35043 Rennes Cedex,1 and

2. Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes UMR215 INRA-CNRS, F-31326 Castanet Tolosan,2France, and

3. Department of Biological Sciences,3

4. DNA Sequencing and Technology Center,4 and

5. Howard Hughes Medical Institute,5 Stanford University, Stanford, California 94305

Abstract

ABSTRACT To facilitate sequencing of the Sinorhizobium meliloti 1021 pSyma megaplasmid, a high-resolution map was constructed by ordering 113 overlapping bacterial artificial chromosome clones with 192 markers. The 157 anonymous sequence tagged site markers (81,072 bases) reveal hypothetical functions encoded by the replicon.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.182.4.1185-1189.2000

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