Targeted Screening for Spontaneous Insertion Mutations in a Lactic Acid Bacterium, Tetragenococcus halophilus-Reference-Cited by-同舟云学术

Targeted Screening for Spontaneous Insertion Mutations in a Lactic Acid Bacterium, Tetragenococcus halophilus

Published:2023-03-29 Issue:3 Volume:89 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Nukagawa Yuya¹,Wakinaka Takura²^ORCID,Mogi Yoshinobu²,Watanabe Jun¹²³^ORCID

Affiliation:

1. Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima, Japan

2. Manufacturing Division, Yamasa Corporation, Choshi, Japan

3. Institute of Fermentation Sciences, Fukushima University, Fukushima, Japan

Abstract

Genetic and strain improvement tools to manipulate a gene of interest are required for the nontransformable lactic acid bacterium Tetragenococcus halophilus . Here, we demonstrate that an endogenous transposable element, IS Teha4 , is transposed into the host genome at an extremely high frequency.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.02005-22

Reference48 articles.

1. Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics

2. Use of Tetragenococcus halophilus as a Starter Culture for Flavor Improvement in Fish Sauce Fermentation

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4. Soy sauce fermentation: Microorganisms, aroma formation, and process modification

5. Isolation of halophilic lactic acid bacteria possessing aspartate decarboxylase and application to fish sauce fermentation starter

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