Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for Clostridium difficile Detection Challenges Cytotoxin B Cell Test and Culture as Gold Standard-Reference-Cited by-同舟云学术

Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for Clostridium difficile Detection Challenges Cytotoxin B Cell Test and Culture as Gold Standard

Published:2011-02 Issue:2 Volume:49 Page:710-711
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Norén Torbjörn¹²,Alriksson Ingegärd¹,Andersson Josefin¹,Åkerlund Thomas³,Unemo Magnus¹

Affiliation:

1. Department of Laboratory Medicine, Microbiology

2. Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden

3. Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden

Abstract

ABSTRACT Compared to the composite gold standard cytotoxin B assay and toxigenic culture, the loop-mediated isothermal amplification (LAMP) test for Clostridium difficile had a sensitivity and specificity of 98%, positive predictive value of 92%, and negative predictive value of >99%. A one-hour turnaround time for the LAMP test provides rapid diagnosis and cost savings.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01824-10

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