Affiliation:
1. Hôpital de Bellevue, F-42055 Saint Etienne,1
2. Hôpital de l'Antiquaille, F-69321 Lyon cedex 5,2 and
3. Hôpital Edouard Herriot, F-69437 Lyon cedex 03,3 France
Abstract
ABSTRACT
CHROMagar Staph aureus (CSAM) (CHROMagar Microbiology, Paris, France) is a new chromogenic medium designed to enable detection of colonies of
Staphylococcus aureus
by their pink color. A total of 775 specimens were cultured in parallel on CHROMagar Staph aureus and conventional media. Among the 267
S. aureus
strains recovered on at least one medium, 263 were isolated on CSAM medium (sensitivity, 98.5%), and 245 (sensitivity, 91.8%) were isolated on conventional media. The specificity of presumptive identification of
S. aureus
on the basis of pink colony color on CSAM medium was 97% (493 of 508). This specificity increased to 100% when coagulase detection with the Staphychrom coagulase test was added and to 98.8% when
S. aureus
surface components were detected by agglutination in the Pastorex Staph Plus test. Susceptibility testing of 67
S. aureus
strains, performed in parallel on pink CSAM colonies and on colonies grown on blood agar, gave similar results. Thus, rapid and accurate recognition and identification of
S. aureus
isolates were achieved with CSAM as the primary isolation medium, followed by the staphylocoagulase Staphychrom test. Antimicrobial susceptibility testing (disk-diffusion method or ATB STAPH System) can be performed directly on pink CSAM colonies.
Publisher
American Society for Microbiology
Cited by
32 articles.
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