Abstract
A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to be selected which resulted in either reversion to Hg2+ resistance (characteristic plasmid R100-1) or sensitivity at a level characteristic of plasmidless strains. Restriction endonuclease EcoRI and BamHI analysis showed that reversion to resistance resulted from loss of TnA from the R100-mer:Tn801 plasmid, whereas the change from hypersensitivity to sensitivity to Hg2+ usually resulted from deletion of part or all of Tn801 plus plasmid deoxyribonucleic acid sequences corresponding to the operator-proximal end of the mer operon.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference13 articles.
1. Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1;Chandler M.;Mol. Gen. Genet.,1977
2. Plasmid cointegrates of prophage lambda and R factor RIOO;Dempsey W. B.;J. Bacteriol.,1976
3. Transposon A-generated mutations in the mercury resistance genes of plasmid R100-1;Foster T. J.;J. Bacteriol.,1979
4. Holmans P. G. C. Anderson and R. C. Clowes. 1978. TnA-directed deletions and translocations within the R6K plasmid p. 38-41. In D. Schlessinger (ed.) Microbiology-1978. American Society for Microbiology Washington D.C.
5. Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid;Lane D.;Mol. Gen. Genet.,1977
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献