Development and Evaluation of Fluorogenic TaqMan Reverse Transcriptase PCR Assays for Detection of Dengue Virus Types 1 to 4-Reference-Cited by-同舟云学术

Development and Evaluation of Fluorogenic TaqMan Reverse Transcriptase PCR Assays for Detection of Dengue Virus Types 1 to 4

Published:2004-12 Issue:12 Volume:42 Page:5935-5937
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ito Mikako¹,Takasaki Tomohiko¹,Yamada Ken-Ichiro¹,Nerome Reiko¹,Tajima Shigeru¹,Kurane Ichiro¹

Affiliation:

1. Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjyuku-ku, Tokyo, Japan

Abstract

ABSTRACT The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.12.5935-5937.2004

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