Affiliation:
1. Departments of Pathology
2. Department of Epidemiology, College of Public Health, University of Iowa, Iowa City, Iowa
3. Medicine, Roy J. and Lucille A. Carver College of Medicine
Abstract
ABSTRACT
The performance of the Etest using Mueller-Hinton agar supplemented with glucose (2%) and methylene blue (0.5 μg/ml) (MH-GMB) for amphotericin B susceptibility testing of 4,936 isolates of
Candida
spp. was assessed against that of Etest using RPMI agar with 2% glucose (RPG). MICs were determined by Etest in both media for all 4,936 isolates and were read after incubation for 48 h at 35°C. The
Candida
isolates included
C. albicans
(
n
= 2,728),
C. glabrata
(
n
= 722),
C. parapsilosis
(
n
= 666),
C. tropicalis
(
n
= 528),
C. krusei
(
n
= 143),
C. lusitaniae
(
n
= 54),
C. guilliermondii
(
n
= 39),
C. pelliculosa
(
n
= 17),
C. kefyr
(
n
= 15),
C. rugosa
(
n
= 11),
C. dubliniensis
(
n
= 5),
C. zeylanoides
(
n
= 4),
C. lipolytica
(
n
= 3), and
C. famata
(
n
= 1). The Etest results with MH-GMB correlated well with those with RPG. Overall agreement was 92.9%, and agreements for individual species were as follows:
C. lusitaniae
, 98.1%;
C. albicans
, 95.1%;
C. glabrata
, 94.3%;
C. krusei
, 91.6%;
C. parapsilosis
, 86.6%; and
C. tropicalis
, 86.4%. The Etest method using MH-GMB appears to be a useful method for determining amphotericin B susceptibilities of
Candida
species.
Publisher
American Society for Microbiology
Reference12 articles.
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