Comparison of an Immunochromatography Test with Multiplex Reverse Transcription-PCR for Rapid Diagnosis of Respiratory Syncytial Virus Infections

Author:

Kuroiwa Yuki1,Nagai Kazushige1,Okita Lisa1,Ukae Susumu2,Mori Toshihiko3,Hotsubo Tomoyuki4,Tsutsumi Hiroyuki1

Affiliation:

1. Department of Pediatrics, Sapporo Medical University School of Medicine

2. Department of Pediatrics, Sapporo Social Insurance General Hospital

3. Department of Pediatrics, NTT East Sapporo Hospital

4. Department of Pediatrics, Tonan Hospital, Sapporo, Japan

Abstract

ABSTRACT A new commercial rapid 10-min one-step immunochromatography (IC) test, SAS RSV test, was compared to another IC test, Directigen EZ RSV, employing RT-PCR as the “gold standard” for detecting respiratory syncytial virus. Of 102 clinical samples, 79 were positive by RT-PCR, 66 (82.5%) were positive with the SAS RSV test, and 55 (69.6%) were positive with Directigen EZ RSV. The specificity of the new test was 91.3% (21 of 23), similar to that of Directigen EZ RSV (100% [23 of 23]). This test performs well enough to be used for patient care.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

1. Falsey, A. R., C. K. Cunningham, W. H. Barker, R. W. Kouides, J. B. Yuen, M. Menegus, L. B. Weiner, C. A. Bonville, and R. F. Betts. 1995. Respiratory syncytial virus and influenza A infections in the hospitalized elderly. J. Infect. Dis.172:389-394.

2. Fouillard, L., L. Mouthon, J. P. Laporte, F. Isnard, J. Stachowiak, M. Aoudjhane, J. C. Lucet, M. Wolf, F. Bricourt, L. Douay, M. Lopez, C. Marche, A. Najman, and N. C. Gorin. 1992. Severe respiratory syncytial virus pneumonia after autologous bone marrow transplantation: a report of three cases and review. Bone Marrow Transplant.9:97-100.

3. Hall, C. B. 2001. Respiratory syncytial virus and parainfluenza virus. N. Engl. J. Med.344:1917-1928.

4. Kelly, J. H. 2004. Advances in the laboratory diagnosis of viral respiratory disease. Pediatr. Infect. Dis. J.23:s6-s10.

5. Kuroiwa, Y., L. Okita, K. Nagai, and H. Tsutsumi. 2003. Detection of respiratory syncytial virus subgroup A and B by multiplex reverse transcription-polymerase chain reaction: comparison with TESTPACK RSV. J. Clin. Pediatr.51:129-133. (In Japanese.)

Cited by 36 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3