Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe-Reference-Cited by-同舟云学术

Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe

Published:2004-01 Issue:1 Volume:42 Page:179-185
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hurtle William¹,Bode Elizabeth²,Kulesh David A.²,Kaplan Rebecca Susan²,Garrison Jeff³,Bridge Deanna²,House Michelle²,Frye Melissa S.⁴,Loveless Bonnie⁴,Norwood David²

Affiliation:

1. Clinical Research Management, North Royalton, Ohio 44133

2. U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701-5011

3. Battelle, Columbus, Ohio 43201

4. Geo-Centers, Newtown, Massachusetts 02459

Abstract

ABSTRACT Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis , Bacillus cereus , and Bacillus thuringiensis . We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis . A real-time PCR assay was developed for the detection of B. anthracis . After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3′ minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.1.179-185.2004

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