False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-Linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified by Western Blotting with Recombinant SARS-CoV Spike Polypeptide-Reference-Cited by-同舟云学术

False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-Linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified by Western Blotting with Recombinant SARS-CoV Spike Polypeptide

Published:2004-12 Issue:12 Volume:42 Page:5885-5888
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Woo Patrick C. Y.¹,Lau Susanna K. P.¹,Wong Beatrice H. L.¹,Chan Kwok-Hung¹,Hui Wai-Ting¹,Kwan Grace S. W.¹,Peiris J. S. Malik¹,Couch Robert B.²,Yuen Kwok-Yung¹

Affiliation:

1. Department of Microbiology, The University of Hong Kong, Hong Kong

2. Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas

Abstract

ABSTRACT Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.12.5885-5888.2004

Reference18 articles.

1. Enzyme-linked immunosorbent assay for detecting antibodies to Borna disease virus-specific proteins

2. Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of Aspergillus fumigatus Aspergillosis

3. Chang, M. S., Y. T. Lu, S. T. Ho, C. C. Wu, T. Y. Wei, C. J. Chen, Y. T. Hsu, P. C. Chu, C. H. Chen, J. M. Chu, Y. L. Jan, C. C. Hung, C. C. Fan, and Y. C. Yang. 2004. Antibody detection of SARS-CoV spike and nucleocapsid protein. Biochem. Biophys. Res. Commun.314:931-936.

4. Chen, X., B. Zhou, M. Li, X. Liang, H. Wang, G. Yang, H. Wang, and X. Le. 2004. Serology of severe acute respiratory syndrome: implications for survival and outcome. J. Infect. Dis.189:1158-1163.

5. Microneutralization test for influenza A and B and parainfluenza 1 and 2 viruses that uses continuous cell lines and fresh serum enhancement

Cited by 75 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Terpenes and cannabidiol against human corona and influenza viruses–Anti-inflammatory and antiviral in vitro evaluation;Biotechnology Reports;2024-03

2. COVID‐19 in early 2023: Structure, replication mechanism, variants of SARS‐CoV‐2, diagnostic tests, and vaccine & drug development studies;MedComm;2023-04

3. Clinical Characterization and Outcomes of Patients with Hypercreatinemia Affected by COVID-19;Healthcare;2023-03-24

4. Recent advances in immunoassay technologies for the detection of human coronavirus infections;Frontiers in Cellular and Infection Microbiology;2023-01-04

5. Retraction for Woo et al., “False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-Linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified by Western Blotting with Recombinant SARS-CoV Spike Polypeptide”;Journal of Clinical Microbiology;2022-11-16