Sensitive and Specific Detection of Yersinia pseudotuberculosis by Loop-Mediated Isothermal Amplification-Reference-Cited by-同舟云学术

Sensitive and Specific Detection of Yersinia pseudotuberculosis by Loop-Mediated Isothermal Amplification

Published:2004-11 Issue:11 Volume:42 Page:5349-5352
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Horisaka Tomoko¹,Fujita Kayoko¹,Iwata Taketoshi¹,Nakadai Aya¹,Okatani Alexandre T.²,Horikita Tetsuya¹,Taniguchi Takahide¹,Honda Eiichi¹,Yokomizo Yuichi³,Hayashidani Hideki¹

Affiliation:

1. Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo

2. School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa

3. National Institute of Animal Health, Tsukuba, Ibaragi, Japan

Abstract

ABSTRACT We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.11.5349-5352.2004

Reference19 articles.

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