New Method for Laboratory Detection of AmpC β-Lactamases in Escherichia coli and Klebsiella pneumoniae-Reference-Cited by-同舟云学术

New Method for Laboratory Detection of AmpC β-Lactamases in Escherichia coli and Klebsiella pneumoniae

Published:2004-10 Issue:10 Volume:42 Page:4799-4802
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Nasim K.¹,Elsayed S.¹²,Pitout J. D. D.¹²,Conly J.¹³,Church D. L.¹³²,Gregson D. B.¹³²

Affiliation:

1. Department of Pathology and Laboratory Medicine

2. Calgary Laboratory Services, Calgary, Alberta, Canada

3. Department of Medicine, University of Calgary

Abstract

ABSTRACT A new cefoxitin-agar medium (CAM)-based assay was compared to the previously published modified three-dimensional (M3D) assay for the detection of AmpC production in Escherichia coli and Klebsiella pneumoniae . Clinical isolates of cefoxitin-resistant E. coli ( n = 5) and K. pneumoniae ( n = 7) and multiple control strains with and without AmpC enzymes were tested by both methods. The CAM method with 4 μg of cefoxitin/ml was equivalent to the M3D method for detecting AmpC production in E. coli and K. pneumoniae . This new method is easier to perform and interpret and allows for testing of multiple isolates on a single plate.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.10.4799-4802.2004

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