Mutations Including IS 6110 Insertion in the Gene Encoding the MPB64 Protein of Capilia TB-Negative Mycobacterium tuberculosis Isolates-Reference-Cited by-同舟云学术

Mutations Including IS 6110 Insertion in the Gene Encoding the MPB64 Protein of Capilia TB-Negative Mycobacterium tuberculosis Isolates

Published:2004-01 Issue:1 Volume:42 Page:390-392
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hirano Kazue¹,Aono Akio²,Takahashi Mitsuyoshi¹,Abe Chiyoji¹

Affiliation:

1. Department of Basic Research, Research Institute of Tuberculosis

2. Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association, Kiyose-shi, Tokyo 204-8533, Japan

Abstract

ABSTRACT A simple immunochromatographic assay, Capilia TB, using anti-MPB64 monoclonal antibodies, is a kit for discriminating between the Mycobacterium tuberculosis complex and mycobacteria other than tubercle bacilli. The sensitivity of the kit was estimated to be 99.2% (381 of 384 samples). The sequencing analysis revealed that all of the Capilia TB-negative isolates had mutations within the mpb64 gene, leading to the production of an incomplete protein as a result of a deletion of the C-terminal region of the protein.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.1.390-392.2004

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