Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri-Reference-Cited by-同舟云学术

Purification and Characterization of Glycerol Dehydratase from Lactobacillus reuteri

Published:1990-04 Issue:4 Volume:56 Page:1195-1197
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Talarico Todd L.¹,Dobrogosz Walter J.¹

Affiliation:

1. Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695

Abstract

A coenzyme B 12 -dependent glycerol dehydratase from Lactobacillus reuteri has been purified and characterized. The dehydratase has a molecular weight of approximately 200,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded a single major band with a molecular weight of 52,000. K m values for substrates and coenzyme B 12 were in the millimolar and the submicromolar range, respectively.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.56.4.1195-1197.1990

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