Transcription of DNA from the 70 S RNA of Rous Sarcoma Virus I. Identification of a Specific 4 S RNA Which Serves as Primer

Author:

Dahlberg J. E.12,Sawyer R. C.12,Taylor J. M.12,Faras A. J.12,Levinson W. E.12,Goodman H. M.12,Bishop J. M.12

Affiliation:

1. Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin 53706

2. Departments of Microbiology and of Biochemistry and Biophysics, University of California, San Francisco, California 94143

Abstract

The enzymatic transcription of DNA from the 70 S RNA of Rous sarcoma virus (RSV) is initiated on the 3′ terminus of a molecule of 4 S RNA which is hydrogen bonded to the viral genome. We labeled this primer with radioactive deoxynucleotides, and demonstrated that its release from 70 S RNA by thermal denaturation was accompanied by a reduction in the template activity of the viral RNA. Two-dimensional electrophoresis in polyacrylamide gels separated the 4 S RNAs associated with the 70 S RNA of RSV into approximately eight fractions, each of which appeared to contain a discrete species of tRNA. The RNA in one of these fractions served as the principal primer for initiation of DNA synthesis by both detergent-disrupted virions of RSV and purified RNA-directed DNA polymerase with RSV 70 S RNA as template.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference31 articles.

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2. Bishop J. M. C. T. Deng A. J. Faras H. M. Goodman W. E. Levinson J. M. Taylor and H. E. Varmus. 1973. Transcription of the Rous sarcoma virus genome by RNA-directed DNA polymerase. In C. F. Fox (ed.) Virus research proceedings of second ICN-UCLA sym- posium.

3. The low molecular weight RNA's of Rous sarcoma virus. I. The 4S RNA;Bishop J. M.;Virology,1970

4. Role of subunits of 60 to 70S avian tumor virus ribonucleic acid in its template activity for the viral deoxyribonucleic acid polymerase;Canaani E.;J. Virol.,1972

5. Evidence for 30-40S RNA as precursor of the 60-70S RNA of Rous sarcoma virus;Canaan E.;Proc. Nat. Acad. Sci. U.S.A.,1973

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