Optimal Detection and Identification of Mycobacterium haemophilum in Specimens from Pediatric Patients with Cervical Lymphadenopathy

Author:

Samra Zmira1,Kaufmann Lea1,Zeharia Avraham2,Ashkenazi Shai2,Amir Jacob2,Bahar Judy3,Reischl Udo4,Naumann Ludmilla4

Affiliation:

1. Department of Microbiology, Rabin Medical Center,1 and

2. Schneider Children’s Medical Center of Israel,2 Petah Tiqva, and

3. Hy-Laboratories, Ltd., Rehovot,3 Israel, and

4. Institute for Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany4

Abstract

ABSTRACT Acid-fast bacilli from pediatric patients with lymphadenopathy were detected in the BACTEC radiometric system and in MB Redox broth, but not on Löwenstein Jensen medium. PCR amplification identified the isolates as Mycobacterium haemophilum , which has special nutrition requirements (iron supplements) for growth. Suitable culture medium ensures optimal recovery of this microorganism, avoiding underdiagnosis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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2. NTM Disease Caused by M. kansasii, M. xenopi, M. malmoense, and Other Slowly Growing NTM;Nontuberculous Mycobacterial Disease;2018-10-06

3. Overview of Non Tuberculosis Mycobacterial Lung Diseases;Mycobacterium - Research and Development;2018-06-20

4. Mycobacterium Nontuberculosis Species;Principles and Practice of Pediatric Infectious Diseases;2018

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