Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin-Reference-Cited by-同舟云学术

Biochemistry of Vibrio cholerae Virulence I. Purification and Biochemical Properties of PF/Cholera Enterotoxin

Published:1970-06 Issue:6 Volume:1 Page:546-554
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Richardson Stephen H.¹²,Evans Dolores G.¹²,Feeley John C.¹²

Affiliation:

1. Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103

2. Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014

Abstract

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Although not completely free from traces of vibriocidal antibody-stimulating activity, the resultant purified toxic immunogen appeared homogeneous as judged by immunoelectrophoresis, immunodiffusion, and disc electrophoresis. Advantages and disadvantages of this purification method are discussed in comparison with those of previously published techniques.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.1.6.546-554.1970

Reference10 articles.

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