Transcriptional Activation of mRNA of Intercellular Adhesion Molecule 1 and Induction of Its Cell Surface Expression in Normal Human Gingival Fibroblasts by Mycoplasma salivarium and Mycoplasma fermentans

Author:

Dong Li1,Shibata Ken-Ichiro1,Sawa Yoshihiko2,Hasebe Akira1,Yamaoka Yuji3,Yoshida Shigemitsu2,Watanabe Tsuguo1

Affiliation:

1. Departments of Oral Bacteriology,1

2. Oral Anatomy I,2 and

3. Prosthetic Dentistry,3 Hokkaido University School of Dentistry, Kita-Ku, Sapporo 060-8586, Japan

Abstract

ABSTRACT Lipoproteins in the cell membranes of both Mycoplasma salivarium and Mycoplasma fermentans were demonstrated to trigger the transcription of intercellular adhesion molecule-1 mRNA in normal fibroblasts isolated from human gingival tissue and to induce its cell surface expression by a mechanism distinct from that of Escherichia coli lipopolysaccharide. The lipid moiety of the lipoproteins was suggested to play a key role in the expression of the activity.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference36 articles.

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2. Distribution of ICAM-1, LFA-3 and HLA-DR in healthy and diseased gingival tissues;Crawford J. M.;J. Periodontal Res.,1992

3. Binding of the integrin Mac-1 (CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylation;Diamond M. S.;Cell,1991

4. A simple technique for eliminating interference by detergents in the Lowry method of protein determination;Dully J. R.;Anal. Biochem.,1975

5. Induction by IL-1 and interferon-γ: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1);Dustin M. L.;J. Immunol.,1986

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