Affiliation:
1. Department of Medicine, University of Washington, Seattle, Washington 98195
Abstract
ABSTRACT
Previous investigations have demonstrated that immunization with
Treponema pallidum
subsp.
pallidum
glycerophosphodiester phosphodiesterase significantly protects rabbits from subsequent treponeme challenge. In this report, we show that the glycerophosphodiester phosphodiesterase amino acid sequence is conserved among 12 strains from a total of five pathogenic treponemes. The invariant nature of this immunoprotective antigen makes it an attractive candidate for inclusion in a universal subunit vaccine against
T. pallidum
infection. In addition, these studies show a silent nucleotide substitution at position 579 of the
gpd
open reading frame which is consistently observed in the non-
T. pallidum
subsp.
pallidum
strains. This sequence alteration introduces a
Ple
I restriction site in the nonsyphilis strains and thus allows genetic differentiation from
T. pallidum
subsp.
pallidum
strains.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference23 articles.
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2. Function and protective capacity of Treponema pallidum subsp. pallidum glycerophosphodiester phosphodiesterase;Cameron C. E.;Infect. Immun.,1998
3. Cameron C. E. C. Castro S. A. Lukehart and W. C. Van Voorhis. Unpublished data.
4. Cameron C. E. C. Castro S. A. Lukehart and W. C. Van Voorhis. Unpublished data.
5. Treponema pallidum major sheath protein homologue TprK is a target of opsonic antibody and the protective immune response;Centurion-Lara A.;J. Exp. Med.,1999
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