Affiliation:
1. Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada
Abstract
ABSTRACT
We investigated the involvement of the CmeABC efflux pump in acquired resistance of
Campylobacter jejuni
to macrolides and tetracycline. Inactivation of the
cmeB
gene had no effect on macrolide resistance when all copies of the target gene carried an A2074C mutation. In contrast, the CmeABC pump significantly contributed to macrolide resistance when two or three copies of the 23S rRNA had an A2075G transition. Inactivation of the
cmeB
gene led to restoration of tetracycline susceptibility in the isolates examined. Complete susceptibility to tetracycline or macrolides, however, was not restored when phenylalanine-arginine β-naphthylamide was used. These data confirm contribution of the CmeABC efflux pump to acquired resistance of
Campylobacter jejuni
to tetracycline and macrolides.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference30 articles.
1. Aarestrup, F. M., and J. Engberg. 2001. Antimicrobial resistance of thermophilic Campylobacter. Vet. Res.32:311-321.
2. Campylobacter jejuni Infections: Update on Emerging Issues and Trends
3. Black, R. E., M. M. Levine, M. L. Clements, T. P. Hughes, and M. J. Blaser. 1988. Experimental Campylobacter jejuni infection in humans. J. Infect. Dis.157:472-479.
4. Blaser, M. J. 2000. Campylobacter jejuni and related species, p. 2276-2285. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), principles and practice of infectious diseases. Churchill Livingstone, Inc., New York, NY.
5. Synergy between Efflux Pump CmeABC and Modifications in Ribosomal Proteins L4 and L22 in Conferring Macrolide Resistance in
Campylobacter jejuni
and
Campylobacter coli
Cited by
66 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献