Rapid Detection of Bovine Mycoplasma Antigens by Counterimmunoelectrophoresis

Author:

Cho Hyun J.1,Langford Edgar V.1

Affiliation:

1. Animal Pathology Division, Health of Animals Branch, Agriculture Canada, Animal Diseases Research Institute (Western), Lethbridge, Alberta, Canada

Abstract

Twelve reference strains of mycoplasma and acholeplasma previously reported to have been recovered from cattle were tested against hyperimmune rabbit serum by counterimmunoelectrophoresis. This technique detected antigen by the formation of precipitin lines with antibody within 1 h and promises to be a useful technique for detecting and identifying mycoplasma isolates in either pure or mixed cultures.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference8 articles.

1. Characterization and classification of bovine mycoplasma;Al-Aubaidi J. M.;Cornell Vet.,1971

2. Bovine mycoplasmas: serological studies by double immunodiffusion, growth precipitation and growth inhibition;Erno H.;Acta Vet. Scand.,1973

3. Bovine mycoplasmas: a serological study by the metabolic inhibition test;Erno H.;Acta Vet. Scand.,1973

4. Rapid detection of Australia antigen by counterimmunoelectrophoresis;Gocke D. J.;J. Immunol.,1970

5. A new mycoplasma species isolated from pneumonic Iungs of calves (Mycoplasma dispar sp. nov.);Gourlay R. N.;J. Med. Microbiol.,1970

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