Role of the Orphan Transporter SLC35E1 in the Nuclear Egress of Herpes Simplex Virus 1

Author:

Maeda Fumio123,Kato Akihisa124,Takeshima Kosuke12,Shibazaki Misato12,Sato Ryota5,Shibata Takuma5,Miyake Kensuke5,Kozuka-Hata Hiroko6,Oyama Masaaki6,Shimizu Eigo7,Imoto Seiya8,Miyano Satoru7,Adachi Shungo3,Natsume Tohru3,Takeuchi Koh3,Maruzuru Yuhei124,Koyanagi Naoto124,Jun Arii124,Yasushi Kawaguchi124ORCID

Affiliation:

1. Division of Molecular Virology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

2. Department of Infectious Disease Control, International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

3. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tokyo, Japan

4. Research Center for Asian Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

5. Division of Innate Immunity, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

6. Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

7. Laboratory DNA Information Analysis, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

8. Laboratory Health Medical Intelligence, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

Abstract

The identification of cellular protein(s) that interact with viral effector proteins and function in important viral procedures is necessary for enhancing our understanding of the mechanics of various viral processes. In this study, we established a new system consisting of interactome screening for the herpes simplex virus 1 (HSV-1) nuclear egress complex (NEC), followed by loss-of-function screening to target the identified putative NEC-interacting cellular proteins to detect a defect in HSV-1 nuclear egress.

Funder

International Joint Research Project of the Institute of Medical Science, University of Tokyo

Japan Agency for Medical Research and Development

MEXT | Japan Society for the Promotion of Science

Ministry of Education, Culture, Sports, Science and Technology

Mitsubishi Foundation

Takeda Science Foundation

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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