Methods for Cultivation of Luminal Parasitic Protists of Clinical Importance

Author:

Clark C. Graham12,Diamond Louis S.12

Affiliation:

1. Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Great Britain,

2. Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, Maryland

Abstract

SUMMARY Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica , Giardia intestinalis , Trichomonas vaginalis , Dientamoeba fragilis , Blastocystis hominis , and Balantidium coli . While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Microbiology (medical),Public Health, Environmental and Occupational Health,General Immunology and Microbiology,Epidemiology

Reference72 articles.

1. Ash, R. L., and T. C. Orihel. 1987. Collection and preservation of feces, p. 5-14. In American Society of Clinical Pathologists (ed.), Parasites: a guide to laboratory procedures and identification. ASCP Press, Chicago, Ill.

2. Avron, B., R. Bracha, M. Deutch, and D. Mirelman. 1983. Entamoeba invadens and Entamoeba histolytica: separation and purification of precysts and cysts by centrifugation on discontinuous gradients of Percoll. Exp. Parasitol.55:265-269.

3. Balamuth, W. 1946. Improved egg yolk medium for cultivation of Entamoeba histolytica and other intestinal protozoa. Am. J. Clin. Pathol.16:380-384.

4. Barret, H. P. 1921. A method for the cultivation of Blastocystis. Ann. Trop. Med. Parasitol.15:113-116.

5. Barret, H. P., and N. Yarbrough. 1921. A method for the cultivation of Balantidium coli. Am. J. Trop. Med.1:161-165.

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