Affiliation:
1. Institute of Microbiology, National Defense Medical Center, Taipei, Taiwan, Republic of China
Abstract
A purified preparation of pyocin B39 was shown to be homogeneous by disc electrophoresis and ultracentrifugation. Pyocin B39 is an antibacterial agent, protein in nature, and is capable of inhibiting the growth of susceptible cells. It caused a susceptible strain of
Pseudomonas aeruginosa
to release ultraviolet-absorbing material. Pyocin B39 appears to be a protein with a high molecular weight; its sedimentation coefficient is 32.8
S
. It was destroyed by 7
m
urea but was resistant to attack by proteolytic enzymes, such as pepsin and trypsin. From its sedimentation coefficient, a molecular weight of about 2 × 10
6
was calculated. An electron photomicrograph of pyocin B39 revealed it as a rodlike particle, 7.50 pm long and 1.08 pm wide, with striations running at nearly right angles to the axes of the particle.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
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