Two Distinct Clones of Methicillin-Resistant Staphylococcus aureus (MRSA) with the Same USA300 Pulsed-Field Gel Electrophoresis Profile: a Potential Pitfall for Identification of USA300 Community-Associated MRSA-Reference-Cited by-同舟云学术

Two Distinct Clones of Methicillin-Resistant Staphylococcus aureus (MRSA) with the Same USA300 Pulsed-Field Gel Electrophoresis Profile: a Potential Pitfall for Identification of USA300 Community-Associated MRSA

Published:2009-11 Issue:11 Volume:47 Page:3765-3768
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Larsen Anders Rhod¹,Goering Richard²,Stegger Marc¹,Lindsay Jodi A.³,Gould Katherine A.³,Hinds Jason³,Sørum Marit¹,Westh Henrik⁴⁵,Boye Kit⁴,Skov Robert¹

Affiliation:

1. National Center for Antimicrobials and Infection Control, Statens Serum Institut, Copenhagen, Denmark

2. Department of Microbiology, Saint Joseph Hospital, Creighton University Medical Centre, Omaha, Nebraska

3. Centre for Infection, Division of Cellular & Molecular Medicine, St. George's, University of London, London, United Kingdom

4. Department of Clinical Microbiology, Hvidovre Hospital, Hvidovre, Denmark

5. Faculty of Health, University of Copenhagen, Copenhagen, Denmark

Abstract

ABSTRACT Analysis of methicillin-resistant Staphylococcus aureus (MRSA) characterized as USA300 by pulsed-field gel electrophoresis identified two distinct clones. One was similar to community-associated USA300 MRSA (ST8-IVa, t008, and Panton-Valentine leukocidin positive). The second (ST8-IVa, t024, and PVL negative) had different molecular characteristics and epidemiology, suggesting independent evolution. We recommend spa typing and/or PCR to discriminate between the two clones.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00934-09

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