Affiliation:
1. Department of Preventive Medicine, University of Washington School of Medicine, Seattle, Washington 98105
Abstract
It is known that plaque formation by some rhinoviruses is greatly enhanced by increasing the concentration of MgCl
2
. The mechanism of this action was studied by investigating the effects of MgCl
2
on rhinovirus HGP adsorption, growth, clumping, thermal stability, and cell susceptibility to viral cytopathic effect. The latent period was at most 7 hr, whether virus was propagated in cells maintained in 0.8 or 30 m
m
MgCl
2
, but virus release was 8- to 310-fold greater in the presence of 30m
m
MgCl
2
, depending on initial multiplicity. Intracellular virus content appeared unaffected by Mg
++
and reached maximal yield (plateau phase) at about 10 hr. Viral adsorption was increased when cells were maintained in 30m
m
Mg
++
. It is likely that the two effects of magnesium, enhanced adsorption and increased virus release, both contribute to enhancement of plaque formation.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
13 articles.
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