Novel Clade of Rickettsia spp. from Leeches-Reference-Cited by-同舟云学术

Novel Clade of Rickettsia spp. from Leeches

Published:2002-02 Issue:2 Volume:68 Page:999-1004
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Kikuchi Yoshitomo¹²,Sameshima Shinya³,Kitade Osamu¹,Kojima Junichi¹,Fukatsu Takema²

Affiliation:

1. Natural History Laboratory, Faculty of Science, Ibaraki University, Mito 310-8512

2. Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan

3. Department of Biology, Graduate School of Arts and Sciences, University of Tokyo, Komaba, Tokyo 153-8902

Abstract

ABSTRACT Intracellular rickettsia-like structures were found in the tissues of a glossiphoniid leech, Torix tagoi , by transmission electron microscopy. Diagnostic PCR analysis using specific primers suggested that of the nine glossiphoniid species examined, two species, T. tagoi and Hemicrepsis marginata , harbored bacteria of the genus Rickettsia . A 1.5-kb eubacterial 16S rRNA gene segment obtained from each of these species was amplified by PCR, cloned, and sequenced. Phylogenetic analysis of the 16S rRNA gene demonstrated that the Rickettsia species found in the leeches constituted a novel clade that is distinct from the clade of arthropod-associated Rickettsia species. In natural populations, 97.7% (43 of 44) of T. tagoi leeches and 100% (9 of 9) of H. marginata leeches carried Rickettsia , suggesting that infection with Rickettsia is prevalent in these leeches. This is the first report of Rickettsia found in annelids.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.2.999-1004.2002

Reference21 articles.

1. Buchner P. 1965. Endosymbiosis of animals with plant microorganisms. Interscience New York N.Y.

2. Chen, D. Q., B. C. Campbell, and A. H. Purcell. 1996. A new Rickettsia from a herbivorous insect, the pea aphid Acyrthosiphon pisum (Harris). Curr. Microbiol.33:123-128.

3. Dasch, G. A., and E. Weiss. 1992. The genera Rickettsia, Rochalimaea, Ehrlichia, Cowdria, and Neorickettsia, p. 2407-2470. In A. Balows, H. G. Truper, M. Dworkin, W. Harder, and K.-H. Schleifer (ed.), The prokaryotes, vol. 3. Springer-Verlag, New York, N.Y.

4. Davis, M. J., Z. Ying, B. R. Brunner, A. Pantoja, and F. H. Ferwerda. 1998. Rickettsial relative associated with papaya bunchy top disease. Curr. Microbiol.36:80-84.

5. Folmer, O., M. Black, W. Hoeh, R. Lutz, and R. Vrijenhoek. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol.3:294-299.

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