Rapid Identification of Probiotic Lactobacillus Biosurfactant Proteins by ProteinChip Tandem Mass Spectrometry Tryptic Peptide Sequencing

Author:

Reid Gregor123,Gan Bing Siang14256,She Yi-Min7,Ens Werner7,Weinberger Scot8,Howard Jeffrey C.14239

Affiliation:

1. Lawson Health Research Institute

2. Departments of Surgery

3. Microbiology and Immunology

4. Hand and Upper Limb Centre, Cell and Molecular Biology Laboratory, St. Joseph's Health Care

5. Pharmacology and Toxicology

6. Medical Biophysics, University of Western Ontario, London, Ontario

7. Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba, Canada

8. Ciphergen Biosystems, Inc., Fremont, California

9. Biochemistry

Abstract

ABSTRACT A novel ProteinChip-interfaced tandem mass spectrometer was employed to identify collagen binding proteins from biosurfactant produced by Lactobacillus fermentum RC-14. On-chip tryptic digestion of the captured collagen binding proteins resulted in rapid sequence identification of five novel tryptic peptide sequences via collision-induced dissociation tandem mass spectrometry.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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