Simple Procedure for Rapid Identification of Vibrio cholerae from the Aquatic Environment

Author:

Choopun Nipa1,Louis Valérie1,Huq Anwar12,Colwell Rita R.12

Affiliation:

1. Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, Maryland 21202

2. Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, Maryland 20742

Abstract

ABSTRACT Biochemical tests commonly used to screen for Vibrio cholerae in environmental samples were evaluated, and we found that a combination of alkaline peptone enrichment followed by streaking on thiosulfate citrate bile salts sucrose agar and testing for arginine dihydrolase activity and esculin hydrolysis was an effective rapid technique to screen for aquatic environmental V. cholerae . This technique provided 100% sensitivity and ≥70% specificity.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference18 articles.

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2. Analysis of 16S-23S rRNA Intergenic Spacer Regions of Vibrio cholerae and Vibrio mimicus

3. Colwell, R. R., and W. M. Spira. 1992. The ecology of V. cholerae, p. 107-127. In D. Barua and W. B. Greenough III (ed.), Cholera. Plenum Medical Book Co., New York, N.Y.

4. Induction of melanin biosynthesis in Vibrio cholerae

5. Duby, A., K. A. Jacobs, and A. Celeste. 1988. Using synthetic oligonucleotides as probes, p. 6.4.1-6.4.10. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology, vol. 1. John Wiley & Sons, New York, N.Y.

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