Affiliation:
1. Physics Department, Loyola College in Maryland, Baltimore, Maryland 21210
Abstract
ABSTRACT
A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference9 articles.
1. Barns, S. M., R. E. Fundyga, M. W. Jeffries, and N. R. Pace. 1994. Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment. Proc. Natl. Acad. Sci. USA91:1609-1613.
2. Bevington P. R. 1969. Data reduction and error analysis for the physical sciences. McGraw-Hill Book Co. New York N.Y.
3. Microbial Diversity in a Hydrocarbon- and Chlorinated-Solvent-Contaminated Aquifer Undergoing Intrinsic Bioremediation
4. Pieper, D. H., and W. Reineke. 2000. Engineering bacteria for bioremediation. Curr. Opin. Biotechnol.11:2626.
5. Press W. H. S. A. Teukolsky W. T. Vetterling and B. P. Flannery. 1992. Numerical recipes in C: the art of scientific computing 2nd ed. Cambridge University Press Cambridge United Kingdom.
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