A Sensitive and Rapid Wastewater Test for SARS-COV-2 and Its Use for the Early Detection of a Cluster of Cases in a Remote Community-Reference-Cited by-同舟云学术

A Sensitive and Rapid Wastewater Test for SARS-COV-2 and Its Use for the Early Detection of a Cluster of Cases in a Remote Community

Published:2022-03-08 Issue:5 Volume:88 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Daigle Jade¹,Racher Kathleen²,Hazenberg Justin³,Yeoman Allan²,Hannah Heather⁴,Duong Diep²,Mohammed Umar¹,Spreitzer Dave¹,Gregorchuk Branden S. J.⁵,Head Breanne M.⁵,Meyers Adrienne F. A.⁵⁶,Sandstrom Paul A.⁵⁶,Nichani Anil⁷,Brooks James I.⁸⁹,Mulvey Michael R.¹⁶¹⁰,Mangat Chand S.¹⁶,Becker Michael G.⁵¹¹^ORCID

Affiliation:

1. Wastewater Surveillance Unit, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

2. Taiga Environmental Laboratory, Department of Environmental and Natural Resources, Government of the Northwest Territories, Yellowknife, Northwest Territories, Canada

3. Department of Municipal and Community Affairs, Government of the Northwest Territories, Yellowknife, Northwest Territories, Canada

4. Department of Health and Social Services, Government of the Northwest Territories, Yellowknife, Northwest Territories, Canada

5. JC Wilt Infectious Diseases Research Centre, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

6. Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Manitoba, Canada

7. One Health Division, National Microbiology Laboratory, Public Health Agency of Canada, Guelph, Ontario, Canada

8. Antimicrobial Resistance Division, Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada

9. Division of Infectious Diseases, University of Ottawa, Ottawa, Ontario, Canada

10. Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

11. Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

Abstract

Wastewater-based surveillance is a powerful tool that provides an unbiased measure of COVID-19 prevalence in a community. This work describes a sensitive wastewater rapid test for SARS-CoV-2 based on a widely distributed technology, the GeneXpert.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.01740-21

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