Author:
Chang L T,Farrah S R,Bitton G
Abstract
Positively charged Zeta Plus filters were used to concentrate enteroviruses from 19 liters of effluent from activated sludge units. Neither the addition of salts nor the acidification of the effluent was required for adsorption of viruses to the filters. Viruses adsorbed to the filters were eluted by treating the filters with a solution of 4 M urea buffered at pH 9 with 0.05 M lysine. Eluted viruses were concentrated into final volumes of 1 to 2 ml by using a two-step concentration procedure that employed inorganic and organic flocculation. Approximately 50% of the viruses added to effluents could be recovered in the final sample. The procedure was used to monitor effluents from activated sludge units at two wastewater treatment plants for the presence of enteroviruses.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference20 articles.
1. Bitton G. 1980. Introduction to enviromental virology. John Wiley & Sons Inc. New York.
2. Recovery of coliphages from wastewater effluents and polluted lake water gy magnetite-organic flocculation method;Bitton G.;Appl. Environ. Microbiol.,1981
3. Bitton G. S. R. Farrah E. M. Hoffmann B. N. Feldberg and Y. J. Chou. 1981. Techniques for virus detection in the marine enviroment. Publication no. 53. Water Resources Research Center University of Florida Gainesville.
4. Effect of a secondary treated effluent on the movement of virus through a cypress dome soil;Bitton G.;J. Environ. Qual.,1976
5. Rotavirus stability and inactivation;Esters M. K.;J. Gen. Virol.,1979
Cited by
29 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献