Trypsinized Placental Cell Cultures for the Propagation of Viruses and as “Feeder Layers”

Author:

Grogan Elizabeth A.1,Enders John F.1,Miller George1

Affiliation:

1. Research Division of Infectious Diseases, The Children's Hospital Medical Center, Boston, Massachusetts 02115

Abstract

The preparation and properties of cell cultures derived from human placental tissue are described and their usefulness for the propagation of animal viruses and as “feeder layers” is indicated.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference6 articles.

1. The serial cultivation of human diploid cell strains;Hayflick L.;Exp. Cell Res.,1961

2. Plaque development and induction of interferon synthesis by RMC poliovirus;Johnson T. C.;J. Bacteriol.,1965

3. Establishment of lines from normal human blood leukocytes by co-cultivation with a leukocyte line derived from a leukemic child;Miller G.;Proc. Soc. Biol. Med.,1969

4. Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propriolactone-inactivated Sendai virus;Neff J. M.;Biol. Med.,1968

5. f : A. Quercioli L. Del Grande P. Cantagalli and V. Bocci. 1968. Virus-induced interferon in human chorionic and placental cell cultures p. 245-254. In International Symposium on Interferons Siena 1967. Academic Press Inc. New York.

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