Detection of Antibodies to Babesia equi in Horses by a Latex Agglutination Test Using Recombinant EMA-1

Author:

Xuan Xuenan1,Igarashi Ikuo1,Tanaka Tetsuya1,Fukumoto Shinya1,Nagasawa Hideyuki1,Fujisaki Kozo1,Mikami Takeshi1

Affiliation:

1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

Abstract

ABSTRACT A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly between sera from B. equi -infected horses and sera from Babesia caballi -infected horses or from normal horses. The LAT results were identical to those of a previously developed enzyme-linked immunosorbent assay. These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference10 articles.

1. Prevalence of equine piroplasmosis in central Mongolia.;Avarazed A.;Onderstepoort J. Vet. Res.,1997

2. Monoclonal antibody against Babesia equi: characterization and potential application of antigen for serodiagnosis.;Avarazed A.;J. Clin. Microbiol.,1998

3. Current state and future trends in the diagnosis of babesiosis.;Bose R.;Vet. Parasitol.,1995

4. Equine piroplasmosis: an update on diagnosis, treatment and prevention.;Bruning A.;Br. Vet. J.,1996

5. A Babesia equi gene encodes a surface protein with homology to Theileria species.;Kappmeyer L. S.;Mol. Biochem. Parasitol.,1993

Cited by 10 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3