Measuring Radioactive Methane with the Liquid Scintillation Counter

Author:

Zehnder Alexander J. B.1,Huser Beat1,Brock Thomas D.1

Affiliation:

1. Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

Abstract

Although a gas proportional counter is the most convenient method of measuring the radioactivity of fixed gases such as methane, it cannot be used when high nonradioactive concentrations of methane are present in the gas phase, due to quenching. If only methane and carbon dioxide are present in radioactive form in the gas phase, a liquid scintillation method for measuring these substances can be used. The procedure is described in detail, and the solubility of methane in liquid scintillation cocktails is determined.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference5 articles.

1. Bray G. A. 1970. Determination of radioactivity in aqueous samples p. 170-180. In E. D. Bransome (ed.) The current status of liquid scintillation counting. Grune and Stratton New York.

2. Anaerobic degradation of benzoate to methane by a microbial consortium;Ferry J. G.;Arch. Microbiol.,1976

3. A new coenzyme of methyl transfer, coenzyme M;McBride B. C.;Biochemistry,1971

4. Rapid method for the radioisotopic analysis of gaseous end products of anaerobic metabolism;Nelson D. R.;Appl. Microbiol.,1974

5. Methane formation and methane oxidation by methanogenic bacteria;Zehnder A. J. B.;J. Bacteriol.,1979

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