Affiliation:
1. Department of Chemistry and Biochemical Sciences, Oregon Graduate Center, Beaverton, Oregon 97005
Abstract
Phanerochaete chrysosporium
produces intracellular soluble and particulate β-glucosidases and an extracellular β-glucosidase. The extracellular enzyme is induced by cellulose but repressed in the presence of glucose. The molecular weight of this enzyme is 90,000. The
K
m
for
p
-nitrophenyl-β-glucoside is 1.6 × 10
−4
M; the
K
i
for glucose, a competitive inhibitor, is 5.0 × 10
−4
M. The
K
m
for cellobiose is 5.3 × 10
−4
M. The intracellular soluble enzyme is induced by cellobiose; this induction is prevented by cycloheximide. The presence of 300 mM glucose in the medium, however, had no effect on induction. The
K
m
for
p
-nitrophenyl-β-glucoside is 1.1 × 10
−4
M. The molecular weight of this enzyme is ∼410,000. Both enzymes have an optimal temperature of 45°C and an
E
act
of 9.15 kcal (ca. 3.83 × 10
4
J). The pH optima, however, were ∼7.0 and 5.5 for the intracellular and extracellular enzymes, respectively.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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