Purification and characterization of Eikenella corrodens type IV pilin

Author:

Hood B L1,Hirschberg R1

Affiliation:

1. School of Biological Sciences, University of Missouri-Kansas City 64110, USA.

Abstract

Eikenella corrodens is a gram-negative human pathogen associated with periodontal diseases and soft-tissue infections. Pilin was purified by association-dissociation and fast protein liquid chromatography; it had an apparent molecular mass of about 14.8 kDa and an N-terminal amino acid sequence reflective of type IV pilins. Antibodies to the purified protein reacted with pili on whole cells. This is the first report of purification of type IV pili/pilin from this organism. Other type IV pili are important virulence factors; we are currently investigating the biological role of pili in E. corrodens.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference37 articles.

1. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. A. Smith J. G. Seidman and K. Struhl. 1987. Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience New York.

2. Piliation control mechanisms in Neisseria gonorrhoeae;Bergström S.;Proc. Natl. Acad. Sci. USA,1986

3. Sequence of fimbrial subunit-encoding genes from virulent and benign isolates of Dichelobacter (Bacteroides) nodosus;Billington S. J.;Gene,1991

4. Central nervous system infections caused by Eikenella corrodens;Brill C. B.;Arch. Neurol.,1982

5. Brinton C. C. J. Bryan J.-A. Dillon N. Guerina L. J. Jacobson A. Labik S. Lee A. Levine S. Lim J. McMichael S. Polen K. Rogers A. C.-C. To and S. C.-M. To. 1978. Uses of pili in gonorrhea control: role of bacterial pili in disease purification and properties of gonococcal pili and progress in the development of a gonococcal pilus vaccine for gonorrhea p. 155-178. In G. F. Brooks E. C. Gotschlich K. K. Holmes W. D. Sawyer and F. E. Young (ed.) Immunobiology of Neisseria gonorrhoeae. American Society for Microbiology Washington D.C.

Cited by 18 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Microbial signatures in human periodontal disease: a metatranscriptome meta-analysis;Frontiers in Microbiology;2024-04-09

2. Virulence of Endodontic Bacterial Pathogens;Endodontic Microbiology;2017-02-11

3. Experimental Methods for Studying Kingella kingae;Advances in Understanding Kingella kingae;2016

4. Eikenella;Bergey's Manual of Systematics of Archaea and Bacteria;2015-09-14

5. Genomic Recombination through Plasmid-Encoded Recombinase Enhances Hemolytic Activity and Adherence to Epithelial Cells in the Periodontopathogenic BacteriumEikenella corrodens;Bioscience, Biotechnology, and Biochemistry;2011-04-23

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3