High-Resolution Genotyping of Chlamydia trachomatis from Recurrent Urogenital Infections-Reference-Cited by-同舟云学术

High-Resolution Genotyping of Chlamydia trachomatis from Recurrent Urogenital Infections

Published:2000-08 Issue:8 Volume:38 Page:3068-3071
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pedersen Lisbeth Nørum¹,Kjær Holger Olaf²,Møller Jens Kjølseth¹,Ørntoft Torben Falck³,Ostergaard Lars⁴

Affiliation:

1. Department of Clinical Microbiology, Aarhus Municipal Hospital,1 and

2. Department of Internal Medicine, Horsens County Hospital, DK-8700 Horsens,2 and

3. Department of Clinical Biochemistry, Skejby Hospital, DK-8200 Aarhus N,3 Denmark

4. Department of Infectious Diseases, Marselisborg Hospital,4DK-8000 Aarhus,

Abstract

ABSTRACT A method for Chlamydia trachomatis restriction fragment length polymorphism (RFLP) analysis and complete sequencing of omp1 was developed for use on samples collected at home, and results were compared. Genotyping by sequencing was superior to RFLP analysis. The omp1 gene in 31 clinical strains harbored few mutations compared to the same gene in ATCC reference strains. Follow-up samples obtained during a 24-week period from 31 patients showed recurrence with the same genotype in five cases and a new genotype in one case.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.8.3068-3071.2000

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