Simple and Reliable Multiplex PCR Assay for Surveillance Isolates of Vancomycin-Resistant Enterococci-Reference-Cited by-同舟云学术

Simple and Reliable Multiplex PCR Assay for Surveillance Isolates of Vancomycin-Resistant Enterococci

Published:2000-08 Issue:8 Volume:38 Page:3092-3095
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kariyama Reiko¹,Mitsuhata Ritsuko¹,Chow Joseph W.²,Clewell Don B.³,Kumon Hiromi¹

Affiliation:

1. Section of Infection Control, Okayama University Hospital, and Department of Urology, Okayama University Medical School, Okayama 700-8558, Japan1;

2. Research and Medical Service, John D. Dingell VA Medical Center, and Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan 482012; and

3. Department of Biologic and Materials Sciences, School of Dentistry, and Department of Microbiology/Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan 481093

Abstract

ABSTRACT Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA , vanB , vanC1 , vanC2/C3 Enterococcus faecalis -specific, Enterococcus faecium -specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.38.8.3092-3095.2000

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