Genetic Elements Carrying erm (B) in Streptococcus pyogenes and Association with tet (M) Tetracycline Resistance Gene

Abstract

ABSTRACT This study was directed at characterizing the genetic elements carrying the methylase gene erm (B), encoding ribosome modification-mediated resistance to macrolide, lincosamide, and streptogramin B (MLS) antibiotics, in Streptococcus pyogenes . In this species, erm (B) is responsible for MLS resistance in constitutively resistant isolates (cMLS phenotype) and in a subset (iMLS-A) of inducibly resistant isolates. A total of 125 erm (B)-positive strains were investigated, 81 iMLS-A (uniformly tetracycline susceptible) and 44 cMLS (29 tetracycline resistant and 15 tetracycline susceptible). Whereas all tetracycline-resistant isolates carried the tet (M) gene, tet (M) sequences were also detected in most tetracycline-susceptible isolates (81/81 iMLS-A and 7/15 cMLS). In 2 of the 8 tet (M)-negative cMLS isolates, erm (B) was carried by a plasmid-located Tn 917 -like transposon. erm (B)- and tet (M)-positive isolates were tested by PCR for the presence of genes int (integrase), xis (excisase), and tndX (resolvase), associated with conjugative transposons of the Tn 916 family. In mating experiments using representatives of different combinations of phenotypic and genotypic characteristics as donors, erm (B) and tet (M) were consistently cotransferred, suggesting their linkage in individual genetic elements. The linkage was confirmed by pulsed-field gel electrophoresis and hybridization studies, and different elements, variably associated with the different phenotypes/genotypes, were detected and characterized by amplification and sequencing experiments. A previously unreported genetic organization, observed in all iMLS-A and some cMLS isolates, featured an erm (B)-containing DNA insertion into the tet (M) gene of a defective Tn 5397 , a Tn 916 -related transposon. This new element was designated Tn 1116 . Genetic elements not previously described in S. pyogenes also included Tn 6002 , an unpublished transposon whose complete sequence is available in GenBank, and Tn 3872 , a composite element resulting from the insertion of the Tn 917 transposon into Tn 916 [associated with a tet (M) gene expressed in some cMLS isolates and silent in others]. The high frequency of association between a tetracycline-susceptible phenotype and tet (M) genes suggests that transposons of the Tn 916 family, so far typically associated solely with a tetracycline-resistant phenotype, may be more widespread in S. pyogenes than currently believed.