The Explosive Human Immunodeficiency Virus Type 1 Epidemic among Injecting Drug Users of Kathmandu, Nepal, Is Caused by a Subtype C Virus of Restricted Genetic Diversity-Reference-Cited by-同舟云学术

The Explosive Human Immunodeficiency Virus Type 1 Epidemic among Injecting Drug Users of Kathmandu, Nepal, Is Caused by a Subtype C Virus of Restricted Genetic Diversity

Published:2000-02 Issue:3 Volume:74 Page:1149-1157
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Oelrichs Robert B.¹,Shrestha Iswar L.²,Anderson David A.³,Deacon Nicholas J.¹⁴

Affiliation:

1. AIDS Molecular Biology Unit,1

2. Siddhi Polyclinic, Kathmandu, Nepal2

3. Hepatitis Research Unit,3 and

4. The National Centre for HIV Virology Research,4 Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria 3078, Australia, and

Abstract

ABSTRACT An explosive epidemic of human immunodeficiency virus type 1 (HIV-1) has been documented among the injecting drug user population of Kathmandu, Nepal, whose seropositivity rate has risen from 0 to 40% between 1995 and 1997. By using Catrimox to preserve whole-blood RNA at ambient temperature for transportation, HIV-1 envelope V3–V4 sequences were obtained from 36 patients in this group. Analysis of the sequences indicated a homogenous epidemic of subtype C virus, with at least two independent introductions of the virus into the population. Viral diversity was restricted within two transmission subclusters, with the majority of variation occurring in V4. Calculation of the synonymous-to-nonsynonymous mutation ratio (Ks:Ka) across this region showed that significant evolutionary pressure had been experienced during the rapid horizontal spread of the virus in this population, most strongly directed to the region between V3 and V4.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.74.3.1149-1157.2000

Reference36 articles.

1. Rapid isolation of HCV RNA from Catrimox-lysed whole blood using QIAamp spin columns;Ali S. A.;BioTechniques,1998

2. Genetic characterization of HIV type 1 from migrant workers in three South African gold mines;Bredell H.;AIDS Res. Hum. Retrovir.,1998

3. Use of dried blood spot specimens in the detection of human immunodeficiency virus type 1 by the polymerase chain reaction

4. Detection of HIV type 1 env subtypes A, B, C, and E in Asia using dried blood spots: a new surveillance tool for molecular epidemiology;Cassol S.;AIDS Res. Hum. Retrovir.,1996

5. Quantification of human immunodeficiency virus type 1 RNA from dried plasma spots collected on filter paper

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