On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli-Reference-Cited by-同舟云学术

On the Process of Cellular Division in Escherichia coli VI. Use of a Methocel-Autoradiographic Method for the Study of Cellular Division in Escherichia coli

Published:1971-10 Issue:1 Volume:108 Page:375-385
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Lin E. C. C.¹,Hirota Y.¹,Jacob F.¹

Affiliation:

1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, and Institut Pasteur and Collège de France, Paris, France

Abstract

A technique allowing chain formation by Escherichia coli for the analysis of the segregation of certain cellular components by autoradiographic methods has been developed. Deoxyribonucleic acid labeled with 3 H-thymidine does not appear to segregate preferentially with one or another of the bacteria. Membrane lipids labeled with either 3 H-oleate or 3 H-glycerol are evenly distributed among all progeny cells. A similar pattern of distribution of cell wall material is observed when 3 H-diaminopimelate is used as the marker.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.108.1.375-385.1971

Reference44 articles.

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