Quantification of Human Cytomegalovirus DNA by Real-Time PCR-Reference-Cited by-同舟云学术

Quantification of Human Cytomegalovirus DNA by Real-Time PCR

Published:2001-02 Issue:2 Volume:39 Page:772-775
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Gault Elyanne¹²,Michel Yanne¹,Dehée Axelle¹²,Belabani Chahrazed¹,Nicolas Jean-Claude³²,Garbarg-Chenon Antoine¹²

Affiliation:

1. Laboratoire de Virologie, Hôpital Armand Trousseau,1

2. EA 2391, UFR Saint-Antoine,2 Paris, France

3. Laboratoire de Virologie, Hôpital Rothschild,3 and

Abstract

ABSTRACT A quantitative real-time PCR assay was developed to measure human cytomegalovirus (HCMV) DNA load in peripheral blood leukocytes (PBLs). The HCMV DNA load in PBLs was normalized by means of the quantification of a cellular gene (albumin). The results of the real-time PCR assay correlated with those of the HCMV pp65-antigenemia assay ( P < 0.0001).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.2.772-775.2001

Reference18 articles.

1. Quantitation of Cytomegalovirus: Methodologic Aspects and Clinical Applications

2. Monitoring levels of human cytomegalovirus DNA in blood after liver transplantation

3. Polymerase chain reaction monitoring reduces the incidence of cytomegalovirus disease and the duration and side effects of antiviral therapy after bone marrow transplantation;Einsele H.;Blood,1995

4. Real time quantitative PCR;Heid C. A.;Genome Res.,1996

5. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase;Holland P. M.;Proc. Natl. Acad. Sci. USA,1991

Cited by 126 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Loopy virus or controlled contortionist? 3D regulation of HCMV gene expression by CTCF-driven chromatin interactions;Journal of Virology;2024-08-30

2. Comparative analyses of DNA extraction methods for whole blood quantification of HCMV DNAemia in patients with hematological diseases: false negative cases in manual method;Heliyon;2023-08

3. T‐lymphocyte subtyping: an early warning and a potential prognostic indicator of active cytomegalovirus infection in patients with sepsis;Immunology & Cell Biology;2022-10-12

4. The host exosome pathway underpins biogenesis of the human cytomegalovirus virion;eLife;2020-09-10

5. Diagnosis of human cytomegvirus association with malignant gliomas and pro- and anti-inflammatories;Current Issues in Pharmacy and Medical Sciences;2020-03-01