Use of BACTEC 9240 Blood Culture System for Detection of Brucella melitensis in Synovial Fluid

Author:

Yagupsky Pablo1,Peled Nechama1,Press Joseph2

Affiliation:

1. Clinical Microbiology Laboratories1 and

2. Pediatric Rheumatology Unit,2 Soroka Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel

Abstract

ABSTRACT Synovial fluid specimens aspirated from patients with arthritis were inoculated into an aerobic Peds Plus blood culture bottle and monitored by the BACTEC 9240 instrument for 4 weeks. A total of 1,072 synovial fluid cultures were processed, and 15 (0.14%) were positive for Brucella melitensis . A single culture, harboring 1.3 CFU of viable organisms per ml, was missed by the instrument. All 14 positive BACTEC cultures were detected within 3 to 7 days.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

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3. Kingella kingae in paediatric patients: 5 cases of septic arthritis, osteomyelitis and bacteraemia;Birgisson H.;Scand. J. Infect. Dis.,1997

4. Use of the BacT/Alert Blood Culture System for Culture of Sterile Body Fluids Other than Blood

5. Five cases of Kingella kingae skeletal infection in a French hospital;La Scola B.;Eur. J. Microbiol. Infect. Dis.,1998

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