Affiliation:
1. Department of Veterinary Biosciences1 and
2. Department of Veterinary Preventive Medicine,2 College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1093
Abstract
ABSTRACT
Ehrlichia chaffeensis
is an obligatory intracellular bacterium of monocytes and macrophages and the etiologic agent of human monocytic ehrlichiosis, an emerging zoonosis. The Lone Star tick (
Amblyomma americanum
) has been implicated as the primary vector of
E. chaffeensis
. The present study examined the sensitivity of the nested reverse transcription (RT)-PCR based on the 16S rRNA gene relative to that of the nested PCR for detection of
E. chaffeensis
in infected DH82 cells, experimentally infected dog peripheral blood mononuclear cells, or experimentally infected
A. americanum
tick samples. The RT-PCR was found to be approximately 100 times more sensitive than the PCR for detection of
E. chaffeensis
regardless of the nature of the specimens. Thus, this RT-PCR is useful for detection of
E. chaffeensis
when a high sensitivity is required. Positive results by RT-PCR also imply the presence of viable pathogens. This is the first demonstration of RNA of
E. chaffeensis
in infected blood and acquisition-fed male, nymphal, and larval
A. americanum
ticks.
Publisher
American Society for Microbiology
Cited by
32 articles.
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