Detection and Analysis of Six Lizard Adenoviruses by Consensus Primer PCR Provides Further Evidence of a Reptilian Origin for the Atadenoviruses

Author:

Wellehan James F. X.1,Johnson April J.1,Harrach Balázs2,Benkö Mária2,Pessier Allan P.3,Johnson Calvin M.4,Garner Michael M.5,Childress April1,Jacobson Elliott R.1

Affiliation:

1. Department of Small Animal Clinical Sciences

2. Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary

3. Department of Pathology, Center for Reproduction of Endangered Species, Zoological Society of San Diego, San Diego, California

4. Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida

5. Northwest ZooPath, Monroe, Washington

Abstract

ABSTRACT A consensus nested-PCR method was designed for investigation of the DNA polymerase gene of adenoviruses. Gene fragments were amplified and sequenced from six novel adenoviruses from seven lizard species, including four species from which adenoviruses had not previously been reported. Host species included Gila monster, leopard gecko, fat-tail gecko, blue-tongued skink, Tokay gecko, bearded dragon, and mountain chameleon. This is the first sequence information from lizard adenoviruses. Phylogenetic analysis indicated that these viruses belong to the genus Atadenovirus , supporting the reptilian origin of atadenoviruses. This PCR method may be useful for obtaining templates for initial sequencing of novel adenoviruses.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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